Three things for miðvikudagur

[nwhiker]

That would be "Wednesday" in Icelandic and I love how it's literally mid-week day, LOL.

1. Perry did what dh calls a speed run of a viral illness this week. He was a bit tired on Monday night, Tuesday he was awful: highish fever (104F, which isn't the highest his has ever been, gulp), body aches, sore throat, the whole nine yards. Note that he's had both flu and Covid vaccines. Anyhow, by the evening, he felt even worse, and his neck was stiff, and his lips numb. I heard that and sent him to the MS clinic. Negative for Covid, negative for flu, and the NP was shocked, she'd expected the flu to be positive considering his symptoms. Today? He's fine. It was a nasty intense 24 hours, but it's apparently over. He stayed home from rowing at least but expects to be back tomorrow. It's not the first time he's done this, gets very sick very fast, but it only lasts a little bit. Ugh.

2. This happened today, as recounted in a text to my spouse:
PI to me: add Nahuel to the animal protocol.
Me: adds Nahuel.
Our animal protocol coordinator: no can do, he hasn't had the proper training.
PI in email to coordinator: please remove Nahuel, he was added in error.
Me, privately: 🤬🤬🤬🤬🤬
I submitted the amendment to the protocol so I'm the one who looks like the idiot.

Also. Last Friday PI tells me we're getting a cell line (that we don't have authorization for) from another researcher on Monday. I scramble to make sure we have the needed supplies on hand (we do, or close enough). I write up a quick change request to our biological use authorization, which won't be reviewed from another month, ugh.

Monday, I went to get the cells, thawed and plated them. By Tuesday they looked good, so I divided them, which eh, needs to happen so we can freeze stock to have as backups. I checked them this afternoon, they should be ready to freeze tomorrow at the earliest, Friday at the latest. Note that freezing these down and keeping one plate to passage for experiments next week is my priority. Note as well that I don't know how these things grow. If they were my usual HEK-293, I could predict doubling time with much more accuracy. These cells are smaller and I have no idea of their growth patterns.

Today PI asks me how the cells are doing because Nahuel needs them by tomorrow. I'm like... Dude. No. I might have some ready by Friday at the earliest, but not tomorrow if I want to have backups. And really... We got them Monday; it's a new cell line etc. No doubt he'll mention to me again that I have almost no experience in cell culture... well, no, maybe not (I mean, beyond a year and a bit, ya know), but I do know one thing: I can't make the cells grow any faster than they are so bug off.

Linnea has a regatta on Saturday and needs to be in Seattle Sunday (more on that in the 3rd thing) so I'll probably stop by the lab and check on the new little guys.

3. Remember last years when Linnea's team were Div II NCAA champions? Well, it's March madness and there are games in Seattle. And the team is going to be honored at the start of one of the games, at the big arena in DT Seattle. Can't be there, so hopefully NCAA will have it online. How exciting for them! They're also, along with the 8 other women's champion rowing teams at WWU going to be inducted into the school's Hall of Fame in early April. Also pretty damn exciting!

Comments

[camelsamba]

Pretty exciting for Linnea and team to be in the Hall of Fame! And to get honored at the game. Very cool.