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I came in yesterday to a note on my desk from my PI explaining how one of the steps in the protocol wasn't necessary. OK, dude, but you keep on telling me that each reaction costs $100 and to be very careful, and now you're telling me to disregard the manufacturer's protocol? Whatever, I guess. Tears of rage in an empty office.

And today just got even worse. I can't detail it all, but basically Elena is a goddess that can do no wrong, and who gets to decide that we need to toss $400 $3000 worth of reagents to buy new ones because she doesn't think the old ones are working. I asked three months ago if I could toss those reagents because I didn't think they were working and was told that no, they worked I was probably just doing something wrong. I'll add that the experiment that I failed at twice? Elena has now failed at twice as well, but for her, it's something wrong with -you guessed- one of our reagents, but me, it's just that I don't know molecular biology well enough. Note that the implication was that I'd done something to the reagent, left it out on the bench or thawed it at 37C or some such. UGH. But Elena was trained by the PI's wife when she worked in the lab, so of course she's perfect. Oh and Elena gets to do work on the neurons one week and the week we don't have pups, she gets to do molecular biology, working "with me" on my project. It's, however, very obvious to me that with the time I have in the lab each week, there is no time for anything other than fucking lab manager work. I've worked two full days and not a bit of time was spent on anything other than the routine tasks, made worse by the fact that people left a mess in the lab for me last Thursday and Friday... namely: emptied all the ethanol bottles, didn't refill. I've pre-diluted the 200 proof stuff, they'd just have to pour it in. Someone left bottles of reagents empty but put away so I didn't notice until I needed one that it was empty, there were discarded gloves all over the place, and oh, someone -the PI I think- broke glassware and left most of it in the sink. Just because I got fucked with the lab manager job does not mean I am the maid. We're all adults. Fucking clean up after yourselves.

There is a very clear division right now: the three of them are the researchers, and I'm the support person. They chat together in a manner that feels like it deliberately excludes me. And often, they speak -of research- in Spanish, completely cutting me out of the conversation. I grew up in a multicultural and multilingual environment. I don't take offense at people speaking a language they prefer even if I do not understand it. However, at work? Make a fucking effort. Also, I'm pretty good at determining if a language is being used because of comfort versus to exclude. This feels like the latter. Or maybe not exclude as much as... I'm not worth the effort of switching to English.

And the killer thing, the last straw that might make me quit? There is a white board by my desk, one of the three or four in the lab. PI told Elena and Nahuel that if they needed something done or bought, they could just leave me a note there. Which Elena promptly did. Way to make me feel like the hired help, not even worthy of an ask, but instead, just write down a list of things to do for the clearly inferior person. For some reason I cannot seem to articulate, that just seems like the final insult, the one that is clearly meant to make it obvious where my place it, at the service and beck and call of everyone else in the lab and certainly not as someone worthy of the respect of a "could you please...?" Up to now, Nahuel has been great about being polite and asking and is getting better at asking in a timely manner (or understanding that I can't make bacteria or cells grow instantaneously!), it'll be interesting to see if this board changes his behavior.

I feel alienated and excluded.

Sorry about the whining. I've spent the last few days in tears more often than not. I hate crying and I hate it even more when it's tears of rage and frustration.
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I don't have access to the program to check if I got it, and I don't remember the exact sequence of amino acids I was trying to delete, but one of my results from DNA sequencing is 18 nucleotides shorter than the others, and that corresponds to 6 amino acids.

The sequence was K-T-X-X-X-W, but I don't remember what the X-X-X were, LOL.

So I have to wait until Monday to find out if my week's worth of work actually paid off.

ETA Whee! It did in fact work. Dh and I went into Seattle to Julefest at the National Nordic Museum, and since we changed buses right in front of my work, we ended up going in on the way back and I checked the results to confirm the deletion and started the overnight culture. This should have been a matter of a few minutes but 1) we stopped at the hospy cafeteria first to get dh some food and 2) I wanted to show him my cells and when I looked at them, they were unexpectedly out of room, grr. So I had to passage them and the whole stop at work for 15 minutes ended up more like a stop at work for an hour and a bit. But having done that, hopefully I can take off early on Wednesday.
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I need to start writing again. Desperately.

But my thoughts are all over the place in a chaotic panicky mess.

If anyone is interested in a better social media platform, bluesky is totally a better place than twitter, LOL. I've had an account for a while, read but did not post there since then, but started transitioning there sometime last month, when twitter got too toxic.

Work is shitty. New postdoc and I'm the one who is supposedly training him. This sucks and again, not what I want to do in a job. Add in that my PI has all of a sudden decided that there must be something wrong with my cell culture technique and is observing and critiquing me as I work with cells. Comments like "you have some theoretical knowledge of cell culture, but not much practice". Dude, I may not have been doing it for twenty years, but a) you haven't done anything in over 5, by your own admission and b) I've been passaging, freezing, thawing, and otherwise dealing with cells for 6 months now, with no contamination and plenty of live healthy cells to show for it. The one experiment that is not working is something else. Not sure what, but I'm 99% sure, as was the previous postdoc -we worked out the protocol and the parameters together and did the experiments in tandem- that is has zip to do with the cells or my sterile technique. I was very close to walking out yesterday. Plus, my dude. You have bigger hands than me. There are manipulations I cannot do without putting down a cap. "Practice," he says, but practice not going to make my hands bigger. Other annoying thing. He gave me a bit of an independent piece of research to do, YAY. He keeps on asking me where I'm at with it. That's nice. The holdup? Purchasing. I'm waiting on the stuff I need to start the experiment, and it took purchasing three days to get a PO to the company. They're fast when it's a simple please order me this product, but when it's paying for a service, it always takes longer and there we are.

I've been avoiding the internet and doing more reading. I like that.

Weight loss is going fine. I'm up a bit these past few days, which is frustrating, but overall, I'm thrilled. While BMI isn't something I trust as, well, indicative of anything, I'm at this point within spitting distance of "normalweight", a status I never thought I'd even approach. We'll see if I get there. These meds are a fucking miracle. Dh's work plan is high deductible, so I'll be paying a lot for them next year until we meet that deductible, but I can afford it and it's totally fucking worth it. Life changing. Not perception changing, though. I still see myself as a fat person but I'm starting to think that other people no longer do. Body dysmorphia is real.

Planning the holidays. Tonight, I'll start the first Tday shopping, and this weekend I'll start to prep the base for the gallon for so of gravy that I make every year... ok, maybe not a gallon, but a lot. I usually end up with plenty for the turkey, and I freeze the rest to use in chicken stews etc over the rest of the year. I'll also get apples to pre-cook for a pie, I'm going to try to make an apple pie this year. I'm terrible with pies. It looks like Christmas is going to be here. My sister will come up and probably bring my mom. We'll celebrate on Christmas Eve and then on Christmas day, get up early, pack brunch, and go to Vancouver to spend the day with my aunt. It'll be her last Christmas. Not thinking about that right now but focusing on the logistics of "a lot to do, lots of food to prep for two holidays, while working, and a house to get in order for guests".

We went to Bend last weekend for four days; I took Friday off. My aunt flew down with a friend of hers, and we had a good time. She's slipping quickly, but still there. My mom is doing SO much better in memory care. I mean, the issues are the same but she's happier, and even not seeing her daily, the slippage in cognitive function in the time between visits isn't at all jarring, in contrast to my aunt, whose is. Sigh. Anyhow. Dementia fucking sucks beyond words.

Funny idea. Dh has done a "twice smoked ham" in the smoker a few times, and his briskets are now legendary in the family, LOL. I am planning fancy roast beef (Perry and I are mulling beef Wellington) for Christmas Eve. I'll need a separate port gravy, so I suggested dh smoke a brisket so I can use the non-smoked tbc trimmings for gravy/demi-glace. Then I came on the brilliant idea of packing and freezing ham and brisket for Anne-Chloe and Linnea as part of their holiday haul. LOL. I think they -especially Linnea- will be thrilled.
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I like that alliteration.

1. Mice. Call me the Grim Reaper. I've going to have to euthanize 12 -at least, possibly as many as 15- cages of mice (three singletons, 12 pairs). All of them -except one cage- are past their breeding age, and we need pups. 😭 And let me tell you. In the era of JD Vance making it clear that post-menopausal women have no societal use beyond taking care of grandkids, euthanizing a bunch of too-old-to-breed mice is kinda adding to the horror of this. I need to find a new job. One without mice or anything else than bacteria, plants, or nematodes. I need to keep this one for a while longer so. The upside is that I have a small bit of research project to do. But mice.

2. The glory and the no-so-glory, LOL. Start with the latter. I bought a pair of leggings from amazon. Size XL, which should have been on the edge of big according to numbers. Too small. I returned and ordered... a pair of XXL, which just fit, they'll work, though after wearing them for an hour, they're weird and I wish I hadn't taken off the tags. I've been consistently wearing Ls in bottoms for a while now, so the XXL both vexed me and made me laugh at myself. In other weight loss news... My weight in kg finally starts with a 6. This is a major major fucking milestone for me, and a number I never ever EVER thought I'd see again. These medications are fucking miraculous.

3. Christmas is turning into a CF. My aunt -who will be availing herself of MAID in March, so this is her last Christmas- was going to come but then decided no, she'd come down to Bend so she can see my mom over November 11th. My brother, who wants to see my aunt and my mom, decided that he didn't want to fly over the holidays either and is planning on coming down early December. Christmas falls on a Wed which means to go to Bend, I'd need to take at least 2 days, and with only having 10 days a year of vacay, using two on a pre-planned trip last April etc, I'm pretty tight on vacation if I want to be able to take off more than a few days next summer. All this is complicated by the fact that my sister has said that if we don't come down for Christmas, she's leaving my mom and taking off for a beach somewhere with her spouse. I just don't know what to do. I drew my line which is my mom spending Christmas alone, so I might just have to have her up here while my sister travels, which is of course complicated by getting her here etc. Ideally everyone would such it up and actually spend the holidays here with me. We could do a Christmas Eve dinner, have brunch and drive up to Canada to spend a few hours with my aunt. But nobody is budging. I don't know what to do. My siblings are usually more flexible than this. Also, corollary about the vacation to the beach blackmail thing. I spent the last year I had free taking care of my mom. I took no vacations for a year (my sister took three or four that year). I had wanted my mom to go to memory care right away because this was clearly not a good solution, but sister insisted that she could come live with her as soon as the remodel was done. Fast forward the remodel is done, my mom is in memory care, which was now and would have been then, the right decision for her, she's doing great. Sister is still taking vacations to far off places and I'm stuck, again having spent the year I had planned on spending doing a lot of travel unable to go anywhere. So grr. Usually I'm on the same page as my siblings but two are retired and two have a lot more vacay than I do.
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Wow, what a freaking week.

I still haven't written up our camping/hiking trip from last weekend because I've been getting home so freaking exhausted.

I walked in Tuesday morning and found the cell incubator was out of CO2 and my go-to people for help were all out. I did find someone to help me and show me how to do it, never having changed one of those before, and it was a good thing: Raul apparently installs those bottles without the plastic "crush" washer, and I could not budge the connection even with the big wrench. Even if I'd known how to safely change the tank, I was not breaking that bolt loose.

Wednesday, I had to euthanize a poor little mousie, though tbh, she was sick (vet report suggested a "neoplasia") and it was the right thing to do, but I don't like doing it. And the bottom of the 4C fridge was a puddle of water. I mopped it up, but it was a bit nerve wracking because there were three plugged in instruments sitting in said puddle of water. I lived! 🤣

Thursday, I had a litter of 11 pups to deal with and found out that we are missing two of the ingredients needed to make more neuronal growth medium, something I hadn't done before. It was last made in March, so I didn't have anything to do with it and I guess Raul didn't order what we'd need to make it again (usually he's pretty good about this). I tore apart the lab looking for two things, even though my July chemical inventory didn't show them. Sigh. So no media to use the neurons but I had to sac the mice anyhow. UGH. I had a little cry... ok, maybe it was a sobbing fit after I finished this. Of course I was there cleaning instruments, red faced and teary eyed, when two people walked into the lab to tag some equipment. I did have the door closed, but one had keys. I will long be grateful to both of them for not saying anything.

Also on Thursday I came in to find that yet again the bacteria incubator had had a hot spell and cooked my bacteria. We need to replace it, I'm getting tired of having to repeat experiments because of this bs. The dept electrical person is supposed to be installing an external digital temperature controller but it's been three or four months and I'm tired of waiting. I mean, the temp is supposed to be at 37C. I try to keep it at about 34C so there is some room for variability, but still, every few days, I come in to a temp above 42C. Blah. I repeated the experiment but since I didn't need the bacteria shaker overnight, left the plates in there with the shaking OFF. Agar plates don't do well shaken, LOL, as one of the undergrads found out when I was doing my MS.

All this to say that it was an exhausting week. And I'm going into a weekend that will be fun, my nephew and niece arrive today, YAY! Then I work three days and we head out for camping at Crater Lake. That trip all needs to be planned and prepped for... I'm not quite sure when.

Dh has been at trains Thursday and Friday so no help from him, he comes home exhausted after that, and he's at trains today too. Tomorrow we're planning on doing fun stuff in Seattle, though of course the "cloudy" forecast for the weekend turned to "pouring rain". 🙄

I'm both wiped and pre-emptively wiped, LOL. I'm good, but... I'm still wiped.
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I have to write up our wonderful quick trip up to around Mt Baker. Camped one night, some great hiking, some gorgeous views, even if the weather wasn't completely cooperative. Tomorrow.

-- Niece and nephew are coming Saturday. SeaTac was hacked a few weeks ago and the visitor pass program isn't up and running. How am I supposed to pick them up? Well, says the airport, talk to the airline. Hello? The airline? The people who've make check-in 100% automatic and don't have live human beings anywhere any longer? Ugh. I'll figure it out, but my visitor pass plan is a no-go and that sucks.

-- That debate. OMG. Harris wiped the floor with him. Ultimately it means nothing, only the votes in a handful of states matter, which really really REALLY sucks, but it was still nice to see. Biden's last chess game was his most masterful, sacrificing himself to set up his replacement. Because most of the (white men) people calling for him to step down? Wanted Newsome or Whitmer (because it's always 'a woman is fine, just not that woman' with them), certainly not Harris. We'll see. Fingers crossed.

-- After the debate, I commented that I needed to make a donation (I did), and dh said, "Yeah, we need to pay for the 90 minutes of pure entertainment."

-- I had to euthanize a mouse today. I'm upset that I didn't notice she was in distress when I checked cages this morning, she was towards the back and while the mice usually come to the front of the cage to check me out, they don't always so I didn't think much of it. Anyhow, that was no fun at all. She was very sick, and I think was in the process of dying even before I turned on the CO2. I hate hate hate doing this. HATE. I whine to dh over text and his response was "Twisp says he'll subcontract for a nominal fee." Ha. As if. One more reason to start looking for another job, though realistically I should stay at this one at least a year if I want to have a prayer in hell of finding anything else.

-- I'm feeling a bit overwhelmed by life right now.

-- Trader Joe's no longer sells any fat free milk. Neither does Costco. Safeway doesn't have any left by the afternoon, same with Target. Whole milk is always plentiful on the shelf. WTF, retailers?
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Ugh. It's been a week. Not all bad, but not much great either.

I got some unexpected results that I knew were a fuck up on my part. The question was how far back did I make the mistake? Turns out at the middle step of five, and I'm pretty sure I can remember what was happening when I did: namely someone coming to talk with me urgently at a point when I needed to pay attention to what I was doing and I swapped two tubes. Blah. All fixed now, but ugh.

And the -20 was full of ice. Last time I needed trypsin, I had to really carefully get it out. Raul was chiseling -not a good idea- some stuff out earlier this week. And then on Wed, we could no longer close the door of the damn freezer without effort. It was time to defrost.



This is the top shelf after I'd pried about half of the boxes that were embedded in the ice out.



It took a lot of puppy pads, warm water, and persuasion. I was able to borrow a freezer for the night so I didn't have to overstuff our other -20. Anyhow long day.

And yes, in case anyone is wondering. That is a mercury thermometer in there. 😐 I need to get rid of it, I shudder at the consequences of it getting broken.

This morning, everything was good, we were at -12, so we transferred everything back. Only to have the temp shoot up to 5. Not good. Note that we'd put in stuff from another -20. It took a while and not opening the door to check the temp every 15 minutes like I wanted to, but it was at -15 when I left so fingers crossed.

My plan this weekend: find a place to buy a small plastic dragon or dinosaur and place it towards the back of the freezer. If the next person to defrost isn't me, hopefully they'll get a grin out of it.

Some of what I do in the lab is standard lab manager crap, which I loathe. Some of it is neuronal preps which means, yeah, killing mouse pups and the cell culture follow through of that. Those neurons go to Raul to do stuff with. Some of what I do is going back and making a systemic catalog of the DNA constructs in the lab, so bench work and database/Excel work, and I'm about half done with that. Some of what I'm supposed to be doing eventually if another department ever gets their act together is some drug screening but that will be almost no bench work (a few hours a week of routine cell culture work at most) and a lot of data analysis at the computer. But here's the deal. Raul has another job (downstairs and he said I can still ask him questions if need be. When the previous lab manager left there was a lot of knowledge lost, but at least Raul had been in the lab for four years and knows a lot). We were supposed to get another post doc staring in October but now it looks like, because of visa issues, that won't happen until next spring. That leaves me a) alone in the lab and b) without enough to do, even if the drug screening thing happens in a timely manner. I have no idea how this is going to work. Unless I can get a project involving neurons, it makes zero sense to maintain mice for over 6 months if we are not going to need a fresh supply of neurons. I don't feel comfortable sacrificing a bunch of mice over that amount of time so I can practice.

All this to say that I think I'm going to start checking out jobs again. I don't love this one enough deal with 6 months in a lab with no real direction and no overarching project beyond sitting at the computer analyzing FRET images.

Blah.
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1. I'm devastated at what the media and some leading Dems did to Joe Biden. And disgusted. Some of them were people that I respected. Others were people I sent money to. I will be more than happy to send money to their primary opponents next time they run. I do love that those disgusting people wanted an open primary at the Convention, and Joe Biden, in a Dark Brandon move, denied them that by endorsing her. It was brilliant.

2. I love Kamala Harris. I supported her in 2020 and was looking forward to supporting her in 2028. I've spent this past week, however, in an up-then-down sort of way. There is a lot of excitement about her candidacy, and it's infectious. But then... I honestly don't think she can win. I think that, like Clinton, she'll blow out the popular vote easy but fail in the Electoral College. I hate this. I think in 2028, after four more years of capable and stable leadership and Trump -either dead or completely mush-for-brains- out of the picture, she could win. This year? I don't think so. They're motivated by hate and they have a systemic electoral college advantage. I think we'll also lose the Senate. By not doing any partisan gerrymandering, NY may have cost us the ability to retake the House and after the Supreme Court's decision about racial gerrymandering the few districts we could have grabbed either in 24 or 26 are in jeopardy. So I'm no longer hopeful about our chances this year, and some of the polls are already scaring me (there is evidence that a) Biden significantly outpolls Harris in some swing states, b) that the most reliable voting block doesn't like her -their mistake, imo-, and c) the never-Trump vote might not follow Harris.) I'll donate and maybe even volunteer and be positive etc when out in public but this -with my two or three readers, and three maybe be an overestimate, 🤣- I'm being honest about my feelings. Ugh.

3. Work is sucking these days. I'm bored.

4. Continuing to plug away at the weight loss thing with Zeppy. That drug is a fucking miracle. I've been eating the same way for over a year now and last fall, I'd pretty much stopped losing more than a few ounces here or there. Zepbound restarted that, I suspect with some biochemical tinkering of my set point, leaving my body no longer defending a very high weight. I've lost more than I ever thought I would or could and I'm less than 10lbs from a dream goal, which might or might not happen: being able to ride Icelandic horses here, right down the road from me! If I get there, I'll be thrilled, but even if I don't, it'll be fine, I'll just have to ride them in Iceland where the weight limit they allow is higher, LOL. I feel so much more relaxed about the day-to-day ins and outs of dieting and eating and exercising. I've always hated and mistrusted the addiction framing on people being fat, for various reasons (*), and at first, I was a bit surprised at the data that show that GLP-1 drugs are effective at helping with addiction. They seem to, which is great and I've seen some commentary about how yeah, fatties are just addicts but... GLP-1 are also being shown to be effective in Parkinson's disease and in a phase 3 trial, Alzheimer's. I don't think we're "there" yet on figuring out exactly how they work and how they help people, though they clearly do. (*) Hard to be addicted to something that you need to actually survive. In addition, the arguments seem very much which came first the chicken or the egg to me. Anyhow, as more research into GLP-1 drugs come out, I hope the addiction model gets ditched wholesale, and hopefully we'll get some insight into the brain biochemistry that is very clearly involved. It always was, as many of us fatties have long suspected, just a little bit more complicated that Calories In Calories Out, which, while try, is elides the whole "how the calories are processed".

5. In light of that, I'll share this review: Trapped fat: Obesity pathogenesis as an intrinsic disorder in metabolic fuel partitioning. Normally I'd steer clear of anything with Gary Taubes's name on it (his agenda has led him down some pretty tortuous paths) but this is nevertheless interesting, though I haven't done enough of a deep dive or search on primary sources to trust it all. Still worth a read, if only to be familiar with this reframing.

6. This is in pink! My baby girl turned 21 this week! OMG, how the eff did that happen, she was just a baby two minutes ago! She's an extraordinary young person!

7. We have a few days of camping booked for late September at Crater Lake. The boat tours will have shut down by then. 😢 Why do they stop those a full 10 days before they close the campground? Still, looking forward to that. And we booked two nights (and a previous one so we can get an early start) at a campground near Mt Baker for early September. Had to book Sunday and Monday night, which was the best I could do for minimizing vacation days. We'll get up early Tuesday morning and hopefully I can get into work in time for a solid half day. Tomorrow, we have to drive Linnea to Bellingham so she can start packing hrr place up, since her old and new places don't overlap, sigh, but we'll be taking her furniture down. Anyhow, plan is to leave her the trailer and head out to go on three short hikes in the area.

8. Perry is leaving for a regatta in Canada tomorrow at 5am. I should get some sleep, we have to drive him to the airport. He'll be pretty close to Niagara Falls, and he's hoping to get a group of athletes together to go visit. I hope he does, and I really want to go back there one of these years, I loved going when I was a kid.

9. Still looking for a plan to get my novel moving towards publication. I know I need an agent, but each time I look at agents/agencies, I realize my poor little book doesn't quite fit into any category. Yes, romance, but it's too long to fit their size requirements. One of these days, I'll figure out my next steps. Maybe I should find someone to put it in audiobook format, since that seems to be a good way to get traction. Anyhow.
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Started this post yesterday (Saturday) but never finished it. Let's see.

Friday at work. I got in early so I could leave early. I found mouse pups overnight, which means my plans for the day would be disrupted by spending the afternoon dealing with them. I had other things to do, but the big thing had to be postponed for that.

I texted this to my extended family group:


Ugh. When the kids were little and I was running them all over the place I used to complain that I had all these chunks of time that were too little to do anything but too big to waste (playing Candy Crush right?) I'm having a bunch of days like that this week. 45 minutes just isn't long enough to do anything productive, I finished all the niggly 15 minute tasks 2 days ago and all I have left is some major work I can't start until 1:45. I have some last minute prep to do, but not til about 1. So I have... about 45 minutes and nothing productive to do. I think I'll print and read a paper, last resort of the bored research scientist, lol.


My SIL:

Lol! Walk outside for fresh air! 😍


This would have been great advice, except that it was 90 degrees outside and just no.

Then, Linnea.

Pull the fire alarm


I laughed.

Tea and a paper. Much less likely to get me in trouble.


Terrible idea, but... there is history there when Linnea pulled a fire alarm many years ago at a NPS lodge. Ah, memories!
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1. My sister visited my aunt, and says that yeah, our impressions are correct: she's slipping a lot faster than my mom did. I have to get up to visit her or figure out a better way to call, she's hard to reach on the phone and evenings really don't work. I'm incredibly sad.

2. This week is dragging. I'm doing BORING stuff at work that has to be done, and as a result, I keep on thinking we're further on in the week than we actually are. I mean, I'd have sworn it was Thursday today, only not.

3. I am so tired of this heat. It's been above 80 (mostly high 80s) for weeks not and it's nasty. When is Fall again?
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Wow, that was a hideous work week to be honest.

Lab inspection by EH&S at the end of the month. Our last -last year, long before I was hired- was well below UW and departmental average and barely out of the "red danger!" zone that apparently means they come back more often to check things out. Needless to say... this is not going to fly.

I mean... Severus Snape may have been my favorite HP character, but the one I most identify with is Hermione Granger.

So. Armed with the three-page check list of things they check for, I started going through the lab. I've been cleaning up since I got there, clearing off benches and making sure that all boxes overhead are behind the shelf "lip". If the Big One hits, it won't make a diff, but in a smaller quake, we at least won't all die killed by a box of 50ml Falcon tubes.

One thing that became obvious this week is that... the chemical inventory needed to get done.

So I did it and it was fucking miserable. I added about 150 entries to our list that had about 220 when I started. I'm not 100% sure I got everything (the -20 freezers had a lot of boxes that I don't think had more chemicals in them), but I got a lot more than was there. There are about 20 chemicals that we supposedly have but I cannot locate in the lab. I'll try to find them again at some point, but if I can't, I'll delete them. Not yet, though. I cheated a bit: I found an entry in the database that I could use (hey, some other lab entered it!) for "Primary and Secondary Antibodies" that had an SDS, so I just said we had about 60 of those, rather than entering each one individually. There are more than 60, but that was a first estimate that I will update. There is really no need to enter each antibody individually.

I should defrost the freezers, but it's too hot to do now. Maybe next winter.

The place where I expect we'll lose the most points is training. I'm up to date and I've asked everyone to catch up on training, but nobody is really doing it. Raul continues to bring food and drink into the lab, sigh, so I'll just tell him to STAY AWAY on inspection day, LOL.

Next week I'll finish labelling all the secondary containers in the 4C, and try and figure out how to realistically label the lines that go from the CO2 cylinders to the incubators.... passing through the ceiling, and try to figure out where the O2 line goes: two lines go behind the biosafety cabinet, only one comes out, so. 😂

So yeah. Long week.
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1. I don't want to go to work tomorrow. Had I thought about it I might have taken the day off. Though I only have a meager amount of vacation days.

2. Talking about meager amount of vacation days... It's absolutely effing ridic that I don't accumulate -me or anyone else with 10 days of vacation a year- a day for one month worked. I mean, come on. So so little vacation.

3. I'd rather have the 5th off so I could stay up with fireworks and a non-existent party until late and not have to worry about getting up tomorrow morning.
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People in gyms (apparently, I don't go to gyms) talk about legs day, and arms day and stuff like that.

Well, I need a hands day.

My hands so dry, they are cracking and bleeding. My nails are a mess. I don't normally care about my nails but they're breaking vertically to below the nail line and that a) hurts and b) catches on ramdom stuff, whch again, hurts.

I'm not supposed to put lotion or hand cream on while in lab and while I've been drinking in the lab (water, really! from a sealed bottle!) for very good reasons (*), I try to sit outside while I eat my yogurt, and I don't use hand cream. (*) Most labs have a table outside where you can leave water bottles and the like. We're at the UW Health Science Building, on a main hallway and anything left out gets stolen. Some other labs in smaller corridors do have stuff out, but we really can't. So my water bottle stays in the lab, in a desk drawer.

Anyhow, all that to say that I don't just have hand cream by my computer to apply as needed, though if this continues, I might say fuck it and do it.

I wear nitrile gloves for lots of things but even then, I'm washing my hands a lot. And the bathrooms are public right, so terrible soap and even more terrible toilet paper. They now have plenty of pads and tampons in all women's (?) bathrooms (yay!) but the fact that women use tp every single time we go to the bathroom does not seem to have moved anyone to supply anything better, LOL. Maybe I'll start a campaign? 🤣

So. Hands a mess. Dry and cracked. I spend the evenings and weekends applying Norwegian Formula hand cream, my fave, but I'm just keeping things from getting worse, it's not getting better.

But I have no idea what do to do give my hands a spa day to help the skin heal and do... something with the nails.
nwhiker: (Default)
1. Wah! I had an hour left on an audiobook and it got snatched away! I won't get it back for at least six weeks. WAH, wah, woe is me. LOL.

2. I suck at mouse handling, and I've been so far deftly avoiding doing any mouse work in the lab. The undergrad research assistant is happily dealing with all of that, I'm deeply impressed with her sang-froid. That said, I have a project that is finally starting to coalesce and I'm quite excited by it.

3. News is good on my brother-in-law, but it's still going to be a sucky few months. We'll be taking my mom the week he has surgery, it looks like dh will have to head down to Portland by himself to pick her up. I think we can give her back, so to speak, the following weekend, but I hope we can do it in Portland because the drive down and up, Saturday and Sunday would be rough. We can't keep her the additional week, because it's a steam weekend at the railway museum for Mother's Day and dh will be at train Thursday, Friday, and Saturday or Sunday, so. Anyhow, we'll all manage, but it's stressful and I really wish my sister had agreed to memory care earlier.
nwhiker: (Default)
Maybe it'll be three things. Maybe it'll be more. I don't know.

1. This past week was... interesting. Worked the full week, mostly doing training. From only mentioning "neuronal cell culturing" I'm now finding out there is a lot of mouse work (*), something he'd mentioned during the second interview, but implied was a lot less than it's going to be, and some work with -no I am not kidding- monkey brains. 😐 (*) Basically, the implication was that I'd have to do some mouse euthanasia. I interviewed for a job that required that a while back and read up a bit on it. I didn't feel totally happy with the idea, but I figured I could euthanize mice with CO2, which seemed to be, from my reading, a common method. Well, no. It's mouse pups with scissors, and yes, I'll have to be trained but I'm not sure even then I've got the guts. So this job may go not further than me crying in front of a case of mouse pups. We shall see. And tbh, I don't think he was trying to hide the info. It's just that he was really enthusiastic about another aspect of the job, as was I, and we were both focused on that. So.

2. White fonted for weight loss Well. Alas, this is bad data collection. First full week on Zepbound, I was up 1lb. Second week, this past week? Down almost 3. Is it the meds, or is it the in a lab all day with only the food I've brought it? It's going to be hard to tell. I think I'm dealing with less "food noise" but is that data or placebo? I probably won't know. But I'll take the weight loss and hope for more!

3. Linnea and team were at a regatta yesterday, she was bow in the Varsity 8, yay her! They came in second, which is fine, and the boat looked really good.

Here are the last 30 seconds or so of the race.

4. Seems like Anne-Chloe is the youngest or one of the youngest lab directors they've ever had at Fred Hutch. Yay, but she still really needs to get a freaking PhD or MD. I've only spent a week at UW and I'm already feeling strangely uneducated and dumb.

5. As if things weren't bad enough... My sister brings my mom up on 14 Mar. She leaves 16 Mar, comes back 30 Mar. 31 Mar is Easter Sunday. We leave Friday 5 Apr for Louisiana before we drive to TX to attempt to see the 8 April Eclipse. This was all going to be a bit stressful what with me being gone all day, but then the folks helping my aunt in Vancouver BC decided that... they want to put the house on the market early April and JoAnn agrees. That's nice. When we suggested those dates, they all said no, we can wait until late spring or early summer. Now it's a crisis to go through everything she has left there and figure out what we want -a lot of her stuff is -thank goodness- at the assisted living place with her, but the last two or three times we were up, everyone (including me) agreed that no, we should wait, it wasn't like the house was going on the market right away. My sister was the only one who'd initially pushed for an earlier time to market and to go through JoAnn's stuff. Now I have no idea what we're going to do. I guess dh and I will have to go up at some point, leave my mom at the assisted living place, and start to weed stuff now. Tbf, the folks up there promised to box of anything we wanted/thought we'd want and store it for us in one of their garages, but really, just fucking no. Sigh.

6. Drove Linnea up to Bellingham today and then went on a hike at the Stimsom Family Nature Preserve. About 3.5 miles on well maintained and graded hiking trail.... in the pouring rain! We looked like drowned rats at the end, but smiling drowned rats! Only iffy moment: tree over the trail. No way to go off trail easier to go around, it was too high for me to get easily over and too long for me to easily get under. I had to do under. At one point, I was essentially in a plank trying to move sideways. At least it was dh, and not the kids... they would have taken video, I'm sure, not to post on Instagram but to show either sibling not present.

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